Cuttlefish Hunting Behavior

From February 2014 to February 2015, I collaborated with the Marine Biology Lab at Woods Hole, USA, to study cuttlefish, an invertebrate marine animal related to octopus and squid, from the perspective of neuroscience. My primary goal was to learn about their behaviour, how to take care of them, and how to modify our lab’s behaviour experiment setups for rats to work with cuttlefish. I took care of cuttlefish in all stages of their life cycle and even developed unique relationships with individuals.

Taking care of the cuttlefish on a daily basis taught me a lot about their behaviour, especially their hunting behaviour. I tried out many different experimental setups to study their hunting behaviour more closely, and learned a lot about arduinos and cameras and how to film sea creatures before I came up with the final design of the Cuttle Shuttle experiment.

Many thanks to Kendra Buresch, Stephen Senft, Alex Schnell, Andrew Carvey, Arthur Petron, Troy McInerney, Kelsey Cramer, Corinne Cramer, Andrea Rummell, George Bell, Alan Kuzirian, Barbara Burbank, Lyda Harris, Dan Calzarette, and Roger T Hanlon for their support and assistance at Woods Hole during this project. Additional thanks to Adam Kampff and the Intelligent Systems lab for enabling and supporting this collaboration.

The behavior paradigm and quantification methods described below open up exciting opportunities to further study aspects of hunting behavior in freely behaving cuttlefish, both in the lab and in the wild. If you are interested in collaborating on research along these lines, I’d love to chat! Please email me at danbee [at] danbeekim.org or message me on twitter @taunbot.

The following write-up of the project is currently being distilled into an academic paper titled “An experimental method for evoking and characterizing dynamic color patterning of cuttlefish during prey capture” (in prep).

Introduction

Abstract

Cuttlefish are active carnivores that possess a wide repertoire of body patterns that can be changed within milliseconds for many types of camouflage and communication. The forms and functions of many body patterns are well known from ethological studies in the field and laboratory. Yet one aspect has not been reported in detail: the category of rapid, brief and high-contrast changes in body coloration (“Tentacle Shot Patterns,” or TSPs) that always occur with the ejection of two ballistic tentacles to strike live moving prey (“Tentacles Go Ballistic,” or TGB, moment). We designed and tested a mechanical device that presented prey in a controlled manner, taking advantage of a key stimulus for feeding: motion of the prey. High-speed video recordings show a rapid transition into TSPs starting 114 milliseconds before TGB (N=114). TSPs are then suppressed as early as 470-500 milliseconds after TGB (p<0.05) in unsuccessful hunts, while persisting for at least 3 seconds after TGB in successful hunts. A granularity analysis revealed significant differences in the large-scale high-contrast body patterning present in TSPs compared to the camouflage body pattern deployed beforehand. TSPs best fit the category of secondary defense called deimatic displaying, meant to briefly startle predators and interrupt their attack sequence while cuttlefish are distracted by striking prey. We characterize TSPs as a pattern category for which the main distinguishing feature is a high-contrast signaling pattern with aspects of Acute Conflict Mottle or Acute Disruptive Pattern. The data and methodology presented here open opportunities for quantifying the rapid neural responses in this visual sensorimotor set of behaviors.

Keywords: behavior, attack, predation, secondary defense, Cephalopod, body patterning, Sepia officinalis, deimatic

Graphical Abstract

Graphical Abstract

Video Summary

Figures and Tables

View the latest drafts of the figures and tables included in the paper.

Supplemental Materials

View the supplemental materials of the paper.

Posters

Presentations and Videos

Below are acrylic models I made to show the main phases of a cuttlefish hunt:

Cuttlefish Hunting Behaviour: At Rest

When a cuttlefish is ‘at rest’, or just hanging out, all of their arms lie flat over their mouth. Their eyes are usually on the sides of their head, in a position optimal for having a 360 degree view all around them. The body pattern on the cuttlefish’s mantle, or back, is optimised to camouflage the cuttlefish and blend into its surroundings.

Cuttlefish Hunting Behaviour: Attention

When a cuttlefish first notices food or prey, it will turn to face the food or prey, and often it will raise its two middle arms.

Cuttlefish Hunting Behaviour: Positioning

As stealth hunters, cuttlefish sneak closer to their prey or let their prey come closer to them, as the cuttlefish hide by camouflaging themselves or burrowing into sand on the ocean floor. The two middle arms often remain raised in front of their face. When the cuttlefish is about one body length away from its food or prey, the next four arms create a barrel to guide and aim their tentacles, the tips of which can become visible during this phase.

Cuttlefish Hunting Behaviour: Tentacles Go Ballistic

When the cuttlefish is ready, it will throw its tentacles towards the food or prey, a moment that we call ‘tentacles go ballistic’ (TGB). The middle two arms are usually still raised, and the next four arms are still formed into a ‘barrel’ to aim the tentacles. During this phase, the bottom 2 arms spread out, as if to create a stabilizing tripod for the moment when tentacles go ballistic. The TGB moment also marks the appearance of the ‘tentacle shot pattern’, a unique, highly fractalated body pattern that appears only in this moment and not at any other time in the cuttlefish’s behavioural repetoire.

References

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